FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22921637
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG96150.043.35829594
TAAGTCG28100.026.42704694
CAAGTCG74950.022.5123494
GAAGTCG110050.017.7664794
TTTTTTC411800.016.6406022
CGAAAAT44400.016.3018041
GTTTTTT302150.016.099621
TATACGC9900.015.6666687
TACGCTA11050.015.3122189
CTCGATT51500.014.6932041
CGGATAT23500.014.61
ATATACG13950.014.15053756
TTTTTTG467050.014.1186162
TTTTTTA413350.013.837912
AAATCCA204300.013.3661284
GCGAAAT32600.013.2638031
GTTTAAT95750.013.2041771
CGCTAAT9650.013.1502591
GAAAAAT221900.013.1108611
CGAATCG17050.012.6803513
Produced by FastQC (version 0.11.5)