FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22093445
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134450.037.54406794
CAAGTCG103300.024.7512194
TAAGTCG31700.021.49842594
GAAGTCG120050.016.16909694
CGCGATT7850.013.1719751
AAAGTCG57650.012.8811894
CGCAAAC45150.012.699891
CGAAGAT85800.012.106061
GGAAAAC146650.011.9222641
CGGAAAT43350.011.8177621
GCCGAAT47100.011.5753721
TTAGACG20500.011.4634148
GTCGTTT19800.011.1565651
GGAAAAT171050.011.1008481
GTTTTAC66900.011.0298951
GTTTTTT250500.011.0135741
TATACGC9450.010.9417997
AATCGTA18500.010.9243245
CGAAAAT44450.010.6794161
GTCGGTT40900.010.5721271
Produced by FastQC (version 0.11.5)