FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21369455
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131200.062.40396594
CAAGTCG112800.033.66666894
TAAGTCG35550.026.83825994
GAAGTCG136100.021.34166194
AAAGTCG47200.018.81991694
TATACGG14950.011.94648834-35
GTTCGAT30050.011.730451
CGTATCG18600.010.8655923
TTCGATT32650.010.7963262
GTCGAAA30500.010.7868861
GTCGGAA38500.010.7428571
GTCGGTT44200.010.739821
GGCGATT40650.010.6371471
GTCGATT22250.010.5617971
GCAAGTC122350.010.44871394
CTCGGTT37800.010.4444451
AAATCGA31300.010.0607044
TCGGATT23300.09.8841212
GTTTATT91800.09.7788681
GTCGAAT12550.09.7370521
Produced by FastQC (version 0.11.5)