FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23017436
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112550.036.20524294
CAAGTCG88700.021.19504294
TAAGTCG28600.019.22727294
GAAGTCG101750.015.93611894
CGCGTAA7600.014.2236831
CGCGAAT10300.014.1456321
GTAAAAC74800.014.0748661
GTCGGTT54000.013.5777781
CGAATCG15350.013.1661243
GTTTATT120000.012.9641671
GTTTTAT162550.012.2885271
AATCGTA23200.012.1551725
AAAGTCG51900.011.95375794
CGCATAT10000.011.751
GTCGGGT47200.011.7499991
CGCGTAT4508.406696E-611.4888891
CTCGTTT38850.011.3719441
GTTCGAT39200.011.2704091
GTTTTCT232300.011.1683171
CTTTAAT106950.011.1622251
Produced by FastQC (version 0.11.5)