FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21757941
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104750.058.77803894
CAAGTCG86300.029.19119594
TAAGTCG31850.026.85714394
GAAGTCG119700.023.44110394
CGCGTAA7050.013.3333331
GTTCGAT24950.012.9979971
AAAGTCG41700.012.96163194
GTCGTTT23850.012.2180291
CGCAAAT26700.011.9700371
CTCGTAT17100.010.9941511
CGCGTTT12950.010.8880311
GTCGATT21300.010.8122061
CTCGGTT35100.010.5783481
CGCGGAT22950.010.4444441
CTCGTTT44150.010.3261611
TTCGATT29300.010.1058022
GTCGGTT41950.09.97139551
TCGGATT26400.09.9696962
GTCGGAT26750.09.8392521
GTCGGAA30750.09.6292681
Produced by FastQC (version 0.11.5)