FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21841787
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101250.053.8004994
CAAGTCG83100.032.69073594
TAAGTCG31900.022.83699294
GAAGTCG110100.021.00272494
CGCTTAA37850.013.53500644-45
AAAGTCG46600.012.70815494
TTAGCGA15100.012.4503318
GCGGTAT23150.012.3844481
CGCGTAT5756.2482286E-912.2608691
GTCGGTT37200.011.751
CGCGGAT20650.011.6077481
GTCCGAT16100.010.8012421
ATCGTAT19550.010.5780046
TCGGATT26250.010.5638082
GGCGAAT42600.010.481221
GGGAAAT159300.010.2969241
CGCTAAT10053.6379788E-1210.2885561
GCCGGTT43500.010.2643671
TCTAAGC71100.010.2461327
GCGAACG15600.010.2435892
Produced by FastQC (version 0.11.5)