FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21841787
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130300.041.48119494
CAAGTCG99850.027.95993894
TAAGTCG32200.023.49999894
GAAGTCG115250.018.59609494
CGCTTAA38500.014.40519544-45
CGACCTA11350.014.0792954
GCGGTAT26850.013.8286771
AAAGTCG52500.013.24952394
CGCGTAA10450.012.1435391
GCCGGTT44500.012.0404491
AATCGTA20100.011.223885
CGGGGTT91150.010.9314321
CGGTATA10103.6379788E-1210.2376232
CACGCTT56600.010.13074142-43
TCTAAGC75750.010.1135317
GGCGATT34850.09.9799131
TCGGATT26200.09.8664122
GGCGGTT45900.09.8300651
GTCCGAT15900.09.7547171
CGGGAAT47600.09.5777311
Produced by FastQC (version 0.11.5)