FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22546544
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123500.055.90526294
CAAGTCG95450.029.1995894
TAAGTCG34150.026.69985294
GAAGTCG144150.021.91051194
AAAGTCG51750.016.07536394
CGAAAAT32300.015.715171
TTTTTTC369150.015.0746322
GTTTTTT302650.015.0480761
CGCGAAT10300.014.1456311
GTTTTAC66550.013.4184831
TTTTTTG406400.012.9411912
GAAAAAT209100.012.5872791
GTTTAAT87400.012.5835241
CGCAATT21600.012.1851851
AAAAATC204250.011.9657282
CGGAAAT45900.011.67320251
AAATCCA189500.011.4089714
TTAGCGA21500.011.3674427
CGCGAAC16150.011.3498451
GCGAAAT28650.011.3193721
Produced by FastQC (version 0.11.5)