FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22546544
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146150.047.40198594
CAAGTCG113300.026.79788294
TAAGTCG34950.023.66809894
GAAGTCG136800.021.54166894
AAAGTCG57750.016.032994
CGAAAAC32300.013.2414861
CGAAAAT42750.012.9730991
GTTTTTT291100.012.8196491
CGGAAAT40400.012.79702951
TTAGCGA15400.012.5129877
CGCGAAT7157.2759576E-1212.4895111
CGCAAAT33100.011.6435051
GGAAAAT154800.011.5071071
GTTTAAT84350.011.4783641
CTCGATT35750.011.0433561
GTTTTAC70450.011.0078071
GTCGATT19350.010.9302331
CTCGGAT60150.010.861181
AATCCAA99800.010.8316635
CTTAGAT48200.010.8236511
Produced by FastQC (version 0.11.5)