FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23994373
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG145500.041.02405594
CAAGTCG103600.025.40540594
TAAGTCG41250.020.73696994
GAAGTCG129050.018.82913694
GTCGGTT43200.012.5115741
AAAGTCG64900.012.16640994
CGAAGAT77500.011.4619351
CGCGTAT5556.4158485E-711.0090071
CTCGTTT47050.010.9883091
TCGGGTC66650.010.9302322
GCGGTAT31450.010.6104931
CGCAAAT32200.010.5093161
CGCGGAT27000.010.4444441
GGAAAAT214950.010.3205391
CGAGAAT39200.010.3112251
TCGGATC49750.010.2974872
GGCGAAT35650.010.2833091
GTTTTTT297900.010.2077871
CTCGGAT79300.010.1349311
GTTTATT133200.010.0915911
Produced by FastQC (version 0.11.5)