FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22678704
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122950.060.0927294
CAAGTCG95100.028.5657294
TAAGTCG42500.027.75764894
GAAGTCG136000.023.60367894
CGCAAAT28600.014.62587451
AAAGTCG56950.014.1123894
CGAGAAC73400.013.6389661
GTCGGTT36900.012.2276421
GTTTTAT161850.011.7318511
GTTTTTT333000.011.5453461
CGAAAAT36900.011.3360431
CGGACAT50800.010.9173231
CGAGAAT37100.010.8948781
GTCGAAC44050.010.8830881
GTTCGAT23450.010.8230281
GTTTATT137500.010.8014551
CGAAGAT83600.010.7942591
GTGAATT131550.010.6469021
GGAAAAT209300.010.5542291
GTTTTAC98600.010.4391481
Produced by FastQC (version 0.11.5)