FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22678704
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG152750.048.61538394
CAAGTCG110800.027.02075894
TAAGTCG43500.024.20230194
GAAGTCG141250.020.86300894
CTCGTTT42250.015.1289941
CGCGTAT5007.748895E-1014.11
CGAGAAC75600.013.6772491
CGAAGAT85550.013.3500881
AAAGTCG67950.013.0036894
CGCAAAT27400.012.6934311
CGCGAAC12150.012.3786011
GTCGGTT34700.012.3256481
CGAATCG15300.012.2875813
CGAGAAT41350.011.9347041
CGAAAAT42550.011.9294941
CGCGTTC24650.011.8215011
CTCGGAT69400.011.7161381
CTTTATT202400.011.4249011
GTTTATT131000.011.409161
GGCGAAT37850.011.0515191
Produced by FastQC (version 0.11.5)