FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21947726
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG96900.052.52941594
CAAGTCG74450.026.3250594
TAAGTCG31550.022.34548494
GAAGTCG112800.018.70833494
GCGAAAT27350.016.6691041
TTTTTTC393550.015.453692
GTTTTTT299700.015.4000671
AAAGTCG45750.014.99890694
CTCGATT46850.014.5464251
CGGATAT23250.014.3526891
TTTTTTG446800.014.1273492
GAAAAAT235850.013.8698331
AAAAATC229000.013.6484722
AAATCGA34400.013.2529074
CTTAGAT72800.012.9766471
AATCCAC113100.012.9239615
CGAAAAC35800.012.8659211
CGAAAAT37450.012.6755681
TTTTTTA416100.012.6733962
GTCGATT17450.012.6590261
Produced by FastQC (version 0.11.5)