FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22337649
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128950.044.7948894
TAAGTCG29700.025.63636494
CAAGTCG97350.025.00873494
GAAGTCG126850.018.5999294
CGAAAAC31650.015.1469191
CGCAAAT30200.013.5397351
AAAGTCG53500.013.52897394
GCGAAAT27400.013.2080291
GTTTTTT288050.013.1511891
CGGAAAT43100.012.9767981
CGAAAAT39700.012.9042841
GTTTAAT85600.012.4088791
TACGCTA9400.011.59
CGCAATT23300.011.2961381
TATACGC8601.8189894E-1110.9302327
GGAAAAT172000.010.8755821
AAATCCA195250.010.6878374
CGCGAAC18350.010.5013621
GTTTTAC71750.010.4153311
CGCTAAT8702.582965E-1010.2643671
Produced by FastQC (version 0.11.5)