FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23221696
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131550.059.3439894
CAAGTCG105200.030.02281694
TAAGTCG36300.026.02479494
GAAGTCG139700.023.24767394
AAAGTCG54450.015.70982694
TATACGC8200.015.4756117
TACGCTA9350.014.0748679
ATATACG12050.013.2614126
CGAAGAT98200.012.2525461
CGGAAAT48950.012.098061
CTTTAAT100550.012.0596721
GTTTTTT301400.012.0228941
GTCGGTT49000.011.8938781
CGAATCG15550.011.7877823
CGAAAAT38600.011.6891191
AATCGGG47750.011.5162325
CGCGAAC16600.011.042171
CGCAATT21900.010.9452061
GTTTATT102150.010.9045521
AAATCGA31550.010.8748044
Produced by FastQC (version 0.11.5)