FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23101846
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG88100.050.0942194
CAAGTCG66750.023.4471994
TAAGTCG28900.020.0034694
GAAGTCG106350.019.66619794
GTCCGAT16100.014.3043481
TCGGATT27100.012.6605172
CTCGGTT37150.011.7658141
GTTCGAT28100.011.3736651
AAAGTCG44750.010.7128594
CGTTCGA16450.010.07
CGCGTAA8109.027644E-99.8641981
CGGGAAT49000.09.7836731
GTCGTTT23750.09.6968421
GGGAAAT169250.09.6360411
GTCGGAT28800.09.6284721
CTCGGAT58200.09.4484541
AATCGTA23400.09.440175
TTCGATT32500.09.42
GTCGGTT45600.09.3793861
ACGTTCG21100.09.355456
Produced by FastQC (version 0.11.5)