FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23101846
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107150.040.9248794
CAAGTCG81350.023.8033294
TAAGTCG28000.020.31071394
GAAGTCG105150.017.11935294
GTTCGAT28000.011.9178561
GTCCGAT15800.011.8987351
CGCGTAA7151.1568773E-911.1748261
CGGGGTT86850.010.3903281
CGCGGAT21350.010.3466031
CGAAGAT86550.010.1548231
TATACGC8351.380613E-910.1317367
TTCGATT32000.09.98752
AATCGTA21950.09.6355355
GGAAAAT212250.09.6103651
TCGGGTC49450.09.5995962
GTCGTTT27250.09.48623851
CTCGGTT37550.09.3874841
CGCTTAA34150.09.35871144-45
GTCCGTT21150.09.3333331
CGCCAAT33350.09.301351
Produced by FastQC (version 0.11.5)