FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23048722
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG159300.047.5015794
TAAGTCG39350.025.32147494
CAAGTCG115350.025.30299294
GAAGTCG138000.021.21811794
AAAGTCG62700.014.54226594
CGCGAAT7650.012.9019611
CGAAGAT78800.012.763961
CGCGAAC16750.012.6268661
TCGAATC19700.012.1675132
CGAAAAT44750.011.7631281
CGCGTAT5605.6908902E-811.751
GTCGGTT41800.011.581341
AATCGGG50500.011.5405935
GTTTTAT148600.011.1016161
GGCGAAT43950.010.907851
CGAAAAC37450.010.7930571
CGCAATT25500.010.6901961
GTTTTTT281650.010.563111
GGAAAAT177200.010.5299091
CGGAAAT45600.010.3070171
Produced by FastQC (version 0.11.5)