FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22679627
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127650.054.6400394
CAAGTCG97650.029.74500794
TAAGTCG33500.027.21790994
GAAGTCG137850.021.30939394
TATACGC9000.017.7555567
TACGCTA9400.016.59
AAAGTCG57000.015.08947494
CGCAAAT34250.013.3109481
ATATACG12850.013.1673156
GTCGATT21050.012.9501181
AATCGGG50900.012.834975
GTCGGTT49700.012.5774651
CAATCGG34600.012.0895964
GTTTTTT302050.011.9036591
GCGGTAT28450.011.8945511
CGAAAAT34000.011.8882361
CGGAAAT45100.011.7760531
CGCGGAT25150.011.3996031
AATCGTA19450.011.1156825
TCGAATC17950.010.9972142
Produced by FastQC (version 0.11.5)