FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22679627
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG153700.045.56278694
CAAGTCG115150.026.81632694
TAAGTCG36300.025.89531794
GAAGTCG134500.020.33754594
TACGCTA9600.015.6666679
TATACGC9800.014.3877557
AAAGTCG62750.013.85657494
ATATACG13100.012.9160316
TTAGCGA19500.012.5333337
TTAGACG24000.012.1416678
CGAAAAT45900.011.8779961
GGCGAAT43100.011.6682141
CGAATCG14850.011.3939393
CGCGAAT12600.011.1904751
CGCAAAC43300.011.1801391
GGAAAAT172800.011.0428241
GTTTAAT82800.010.9553141
GTCGTTT19350.010.9302321
CGAAGAT80000.010.92751
CGGAAAT48950.010.7538311
Produced by FastQC (version 0.11.5)