FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22660593
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG153250.043.3350794
CAAGTCG114450.024.55744794
TAAGTCG33400.021.6706694
GAAGTCG130750.019.19541294
AAAGTCG56750.014.07929494
GTCGGTT43650.013.7823591
CGAATCG15800.013.0886073
TATACGC7300.012.8767127
CGCGATT6955.638867E-1112.1726621
CGAAAAT42900.012.051281
CGCAAAT34100.011.7155411
ATATACG11350.011.5947136
CGCGTTT14800.011.4324321
CGCGTAT5455.0731614E-711.2110091
GGAAAAT159200.011.189071
AATCGGG44750.011.1329615
CGCGAAC12250.011.126531
CTCAGAT174200.011.1159581
CGCAATT22900.010.8777281
CGGAAAT46250.010.7718911
Produced by FastQC (version 0.11.5)