FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22382931
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127600.040.77507894
CAAGTCG98850.025.91299894
TAAGTCG27350.022.51188394
GAAGTCG120450.016.42756594
CGCGTAT4051.7098137E-713.9259261
GTCCGAT14600.013.1986291
CGCTTAA31450.011.95548544-45
AATCGTA18700.011.5614975
CGTTCGA24000.011.3583347
CGTATCG18800.011.253
GTTCGAT38600.010.8367881
AAAGTCG55150.010.65276494
TCGCATA7955.8935257E-1010.641517
CTCGGTT36750.010.4870751
CGCGTAA8159.058567E-1010.3803671
TTAGCGA21050.010.2707838
GGCGGAT90000.010.131111
GGCGGTT57100.010.1243431
GCGGTAT27500.010.0836361
TATACGG16450.010.034-35
Produced by FastQC (version 0.11.5)