FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22556552
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120200.050.51913594
CAAGTCG92400.028.07792394
TAAGTCG33950.022.70397694
GAAGTCG137400.019.2583794
AAAGTCG47650.013.90765994
GTTTATT88600.013.3148981
CGAATAT11500.013.078261
GTCGAAT12900.012.7519381
CGAAGAT87250.012.2280791
GTTTTTT243550.012.1962621
CGAAAAT31650.012.1769351
TATACGC8600.012.0232557
CGCGGAT28700.011.9547031
GTTTTAT123900.011.7974171
CTTTATT153900.011.7576341
AAATCGA27800.011.6654674
CGCAAAT30000.011.5933331
AATCGTA20800.011.5240375
CTTTAAT93650.011.3924181
CGCAATT22500.011.281
Produced by FastQC (version 0.11.5)