FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22556552
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG145850.040.63558294
CAAGTCG104050.024.84382494
TAAGTCG35200.022.0312594
GAAGTCG136400.018.53812494
CGGAAAT44000.012.7113631
CGAAGAT90550.011.8343451
GTCGGTT38550.011.8261991
AAAGTCG57900.011.68911894
GTTTTAT118500.011.6607591
CGAATCG16000.011.4562493
GGAAAAT163400.011.3904531
CTTTATT140150.011.3014631
CGCGAAC13200.011.0378781
CGAATAT13650.011.0183141
CGCAATT22200.011.0090081
GTTTATT96150.010.9006751
GTCGGAT37750.010.83178794
CGCGAAT17800.010.8258431
TCGAATC16950.010.8141592
GTTTTTT241650.010.8139861
Produced by FastQC (version 0.11.5)