FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23106184
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG82500.040.79030294
CAAGTCG67050.023.4123894
TAAGTCG27400.020.58394294
GTTTTTT292550.016.3869421
TTTTTTC401900.016.1266482
TATACGC7900.016.0632927
TACGCTA9250.015.751359
GAAGTCG105950.015.70363294
TTTTTTG456250.014.5146292
CGCAAAT28850.014.0103971
GAAAAAT234150.013.8500951
CGGATAT24100.013.8464731
GTTTTAC67000.013.6791031
CGAATCG17950.013.353763
CGAAGAT89350.013.0979291
GGAAAAT171400.013.0250871
CGAAAAT39050.012.878361
TTTTTTA400150.012.6852422
GTTTAAT97050.012.6398751
AAAAATC218750.012.5691422
Produced by FastQC (version 0.11.5)