FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21659665
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116800.060.9631994
CAAGTCG98450.031.4606494
TAAGTCG31500.025.51428694
GAAGTCG126250.020.47524894
AAAGTCG45750.016.64262494
GTTCGAT28000.013.9321431
GTCGGTT42550.012.8131621
ATCGTAT18800.012.0000026
GTCGAAT13200.011.393941
CGCGTAA6352.7135684E-811.1023631
GGCGAAT46050.011.0228021
AATCGTA23750.010.8842125
CGCGAAT5304.41474E-610.6415111
TTCGATT30900.010.4951472
GCGGTAT22150.010.3972921
GTCGTTT20900.010.3444981
CGGGAAT50950.010.3316971
CGCGTAT4751.8314325E-49.8947371
GTTTATT94950.09.7019481
GGGAAAT146500.09.6245751
Produced by FastQC (version 0.11.5)