FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22934868
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG129250.043.0909194
CAAGTCG105700.023.29990694
TAAGTCG30500.022.80655994
GAAGTCG121400.019.97693694
GTTTTTT311750.013.9153171
TCGAATC19700.013.83756352
CGAAAAC31750.013.6188981
GCGAAAT26150.013.3001921
AAAGTCG52300.013.30019294
CGAATCG15100.013.0728483
CGCAAAT32550.012.7066061
CTCGATT36850.012.4993221
GGAAAAT174500.012.4435551
AAATCCA203200.012.4207694
CGAAAAT42800.012.4088781
CGCGTTT15800.012.1962031
AAATCGA37000.012.1945954
CGCAATT20150.012.1290331
CGGAAAT43600.012.0733961
AAAAATC212800.011.9266932
Produced by FastQC (version 0.11.5)