FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22231419
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114550.056.17023594
CAAGTCG87500.029.38171494
TAAGTCG37450.026.35513994
GAAGTCG131700.022.19741894
GTTTTTT299050.014.8363151
AAAGTCG49300.014.30020294
CGAAAAT33200.014.0150611
TTTTTTC378600.013.7921292
GTTTAAT94450.013.0873481
CGAAAAC30850.012.7974061
GTTTTCT213950.012.3458751
GTTTTAT166600.012.0462191
GTCGATT19950.012.0150381
TTTTTTG422700.011.9195642
CGGATAT23550.011.7749461
GAAAAAT215750.011.7200461
GGAAAAT168900.011.4647721
AAAAATC211200.011.4161932
CGGAAAT45750.011.4032791
CTCGATT34400.011.34011651
Produced by FastQC (version 0.11.5)