FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23334715
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114500.050.20174894
CAAGTCG85550.025.98597394
TATACGC9900.020.4141437
TACGCTA11100.020.3243249
GAAGTCG133600.019.55988194
TAAGTCG32300.019.3529494
ATATACG13300.015.9022556
AAAGTCG46300.015.22678294
GTTTATT92400.013.7337671
GTTTTAC59850.012.9573941
CTTTATT164750.012.4952971
CGAAGAT92900.012.2938641
GGCGAAT79850.012.1840951
CGCAAAT33550.012.047691
GTTTAAT82850.012.0265541
TTTTTTC344000.012.0095932
CTTTAAT90650.011.9249871
GTTTTTT264750.011.6634561
CGAATCG14650.011.5494883
GGAAAAT161750.011.3904171
Produced by FastQC (version 0.11.5)