FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22188944
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG76250.014.42360794
TTAGCGA20800.012.8798078
TAGCGAG23900.012.7824279
CGCGTAT4455.377133E-712.6741571
GTTCGAT29950.012.5542561
CGGGAAT51250.012.0136581
CGGAAAT43400.011.0460831
GTCGTTT23450.011.0234541
TATACGG16850.010.73887334-35
TTCGATT31950.010.5915492
CTCGGTT40250.010.5093171
GTTTATT119350.010.1994131
TAAGTCG17650.010.1189894
GTCGTAT10355.456968E-129.9903381
GTCGGTT44050.09.8161181
GGGAAAT152650.09.7910261
CGCGAAT9651.6552804E-109.74093151
CGAAGAT87600.09.7111871
GGCGATT36700.09.6049041
CAAGTCG55650.09.4591294
Produced by FastQC (version 0.11.5)