FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22877794
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108300.051.73037394
CAAGTCG83450.024.89394894
GAAGTCG118500.021.93333294
TAAGTCG34200.020.7514694
GTTTTTT323200.015.9235761
AAAGTCG47700.014.58280894
TTTTTTC426200.014.391132
GCGAAAT27550.013.4773131
CGAAAAT34250.012.7620421
GTTTAAT104100.012.3707971
GAAAAAT250400.012.3506381
GTTTTTC192100.012.2821441
AAATCGA34450.012.1422344
CGGAAAT49400.011.8927131
CGCGAAC14650.011.8703061
AAAAATC244400.011.6730762
AAATCCA224400.011.4986634
GGAAAAT199600.011.4674351
CGAATCG16400.011.4634143
GCCGAAT48350.011.2761111
Produced by FastQC (version 0.11.5)