FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22020376
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG78550.025.07065494
CAAGTCG63550.015.30920494
CGCGTAA5701.8189894E-1214.8421041
CGCGAAT7450.014.5100661
GTCGTTT23800.014.02100851
AATCGGG44300.013.261855
CGAAGAT90800.013.0440531
GTTTATT121050.012.9293681
TAAGTCG24850.012.48289794
GTCGGTT48700.012.3531821
CGCGTTT14850.012.3434341
GTTTTAC76750.012.2475571
CGAAAAT47900.011.9707721
CAATCGG35050.011.9343794
AATCGTA18650.011.8445035
GTTTTAT152100.011.4641671
CGAATCG15250.011.4032783
GTCCGAT13400.011.223881
TCGGATC38400.011.13802052
CTCGGAT57750.010.9870121
Produced by FastQC (version 0.11.5)