FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23186441
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119200.033.6333994
CAAGTCG90850.021.67639294
TAAGTCG27400.020.06934494
GAAGTCG109400.015.68098894
AACGTCG27750.013.5495499
GTAACGT27300.013.256417
GTTTTTT289800.013.2339541
TTAGCGA25050.012.9461087
TAACGTC29500.012.7457638
CGCGAAC18850.012.7161811
CGAATCG17050.012.6803523
GTCGGTT44300.012.5191871
CGAGTAA30050.012.3560734
TAGCGAG26350.012.30748
CGAAAAT47700.012.2180291
CGAAGAT87650.012.17227651
GGAAAAC155750.012.130981
CGCGAGT30050.011.4176371
CGCAAAT33900.011.36873151
CGCGTTT15800.011.3037981
Produced by FastQC (version 0.11.5)