FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22203775
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106200.051.55837694
CAAGTCG82850.027.28666394
TAAGTCG30700.023.27035794
GAAGTCG124050.017.99677594
GTCGGTT44550.014.5589231
AAAGTCG48250.014.51398994
CGAAAAT33300.012.420421
CGCGAAC17550.012.3190871
GTTTAAT82750.011.5867071
GTTTTAC64650.011.4864651
GTTTATT95500.011.22094251
CGCAAAT29150.011.1252141
GTTTTTT247550.011.087861
GTTTTAT140100.011.0035691
AAATCCA185800.010.9278784
GGAAAAT168950.010.8215441
CTCAGAT156350.010.6715711
CGGAAAT43350.010.6251431
GCGGTAT21750.010.5885051
CGAAGAT91850.010.4899291
Produced by FastQC (version 0.11.5)