FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22166024
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAT25100.018.1633471
AAGTCGG48400.014.08057894
TTAGCGA24900.012.83534058
GTCGGTT49550.012.4258331
TAGCGAG29150.012.4150949
TTCGATT31300.012.1629392
TATACGG18900.011.68783134-35
CGGGAAT55000.011.281
GTCGTTT22650.010.7902871
GTATACG17250.010.48985534-35
GTTTATT103950.010.4896581
GTCGGAG38750.010.4309671
TCGATTC33400.010.4131743
CGTATCG15850.010.3785493
CGGGGAT80300.010.1257781
GGCGTTT64450.09.8448411
GTCGGAT26550.09.7363461
CGCGTTT15650.09.6102241
GTCGCGT15850.09.4889591
CGCAAAT26950.09.4174391
Produced by FastQC (version 0.11.5)