FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22277828
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG49400.017.22064894
GTTTTTT318800.015.8779791
TTTTTTC432900.014.635252
GCGAAAT30150.014.0298511
GTTTAAT106800.013.8183531
GAAAAAT247450.013.4665581
CGCAATT21050.013.3966761
CGCAAAT34500.013.3507251
AAATCGA35750.013.2783224
CTCGATT49200.013.1829261
TTTTTTG468650.013.1477642
AAATCCA213950.013.0488424
TTTTTTA453750.012.9580162
GGAAAAT184600.012.7556881
AAAAATC248000.012.65967752
CGAAAAC37300.012.4745311
GTTTTAT183150.012.4460821
CGAGATT52400.012.3778632
CTTAGAT74150.012.2333121
CTCGAAT39850.012.1480551
Produced by FastQC (version 0.11.5)