FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22277828
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT323000.015.2495361
TATACGC9600.014.68757
AAATCGA31850.014.4615384
CGAAAAC38300.014.3577021
CGGATAT23000.014.3043491
CGCAAAT30150.013.8739631
CTCGATT47400.013.78271
TACGCTA7850.013.77079
CTTAGAT83100.013.2346571
CGCAAAC40550.012.9815051
GTTTTAC75350.012.7869931
GAAAAAT221950.012.4726291
AAATCCA207400.012.3958544
CGCGTTT14050.012.3772251
AAAAATC227800.012.3380162
ATATACG13350.012.3220986
CGGAAAT41000.012.0365861
CTCAGAT171400.011.8733951
GTTTAAT97450.011.8645471
GCGAAAT26800.011.5746261
Produced by FastQC (version 0.11.5)