FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22162112
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115300.054.541294
CAAGTCG96900.029.97523194
TAAGTCG36200.027.00552494
GAAGTCG133550.021.6435894
AAAGTCG49450.018.05864594
TATACGC8550.014.29239757
AACGTCG25350.013.7199219
CGAAAAT34950.012.9098711
GTTTTTT304900.012.8560191
TTTTTTC391600.012.7941782
GTCGAAT14800.012.70270251
AGTAACG28550.012.5113836
GTAACGT28550.012.346767
CGAGTAA28150.012.1882774
TTCGATC21800.011.8577992
TAACGTC31000.011.5225818
CGCGTAT4901.7218754E-611.5102041
GTTTTAC67100.011.4172881
TACGCTA8651.8189894E-1211.4104059
GGAAAAT175200.011.4012561
Produced by FastQC (version 0.11.5)