FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22162112
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG135250.046.42661794
CAAGTCG106650.028.86544694
TAAGTCG33300.027.5225294
GAAGTCG128500.022.05525294
GTTTTTT297400.012.6112991
AAAGTCG57450.012.59878294
CGGAAAT42700.011.5573781
CGCGTTT15950.011.4921631
GGAAAAT176800.011.1917431
CTTTAAT99600.010.9949811
GGAAAAC153750.010.8520331
GTTTTAC69200.010.7312151
GTCGAAT14150.010.6289761
CGAAAAT42300.010.4444451
CGAAGAT79050.010.4048071
GTTTTTC173150.010.0161711
GTTTTAT151550.09.9861431
GCGAAAT28050.09.8859191
CGAAAAC36700.09.8610361
CGCATAT9551.3460522E-109.8429321
Produced by FastQC (version 0.11.5)