FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22724411
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108350.051.5763794
CAAGTCG89050.029.18697594
TAAGTCG30050.020.80199694
GAAGTCG112750.017.79955794
AAAGTCG51250.012.6556194
CGCTTAA37500.012.28266644-45
CGCGTAA9350.012.0641721
AATCGTA24200.012.0413225
GGCGAAT54900.011.8998171
CGCGTAT5303.5355697E-711.5283021
GTCGGTT46250.011.3816211
GTCGGAG35350.011.3012731
TCGGATT29000.011.1827582
CGGGGTT94650.011.0237731
ATCGTAT20150.010.962786
CGCGGAT24750.010.8242431
GTCGGAT32650.010.7963241
GTCGGGT46950.010.7113951
GCCGGTT52600.010.633081
GTCCGAT15950.010.3134791
Produced by FastQC (version 0.11.5)