FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22066081
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114050.033.62735794
CAAGTCG80750.020.48792894
TAAGTCG27900.018.19354894
GTTTTTT308850.015.4155751
AAATCGA35200.014.9545454
GAAGTCG107300.014.06057894
CGCAAAC40100.013.7132171
CTCGATT45600.013.2960531
CTTAGAT71000.013.2394361
CGAAAAC39450.013.2243351
TACGCTA11450.013.1353729
GCGAAAT25000.012.9720011
CGGAAAT43900.012.8473811
CGAATCG15450.012.77673
AAATCCA206350.012.7322514
TATACGC10800.012.6203727
AAAAATC215400.012.5464262
CGAAAAT39550.012.359041
CCCGAAC49250.012.2152291
CGCAAAT31200.012.2019231
Produced by FastQC (version 0.11.5)