FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22347208
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG121400.057.99505694
CAAGTCG92250.029.14254894
TAAGTCG34750.027.45611494
GAAGTCG143650.022.8701794
AAAGTCG51650.019.01839394
TTTTTTC348650.014.9634292
GTTTTTT273100.014.9381191
CGAAAAT36300.013.8539951
CGGAAAT48350.012.3453981
TTTTTTG380700.012.3333332
GTTTTCT203300.012.1372361
CGCGAAC20750.012.0048191
GAAAAAT207800.011.964871
AAAAATC200850.011.7470752
CTCGATT36400.011.6208791
GGAAAAT170900.011.6056181
GTTTTTC146650.011.4735761
CGAATCG16850.011.4362033
GTTTAAT84150.011.393941
GTTTTAT151950.011.0115181
Produced by FastQC (version 0.11.5)