FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22768890
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG113450.053.35918894
CAAGTCG87850.030.01366294
TAAGTCG30100.023.26578194
GAAGTCG135850.020.9657794
AAAGTCG51700.015.00000194
CGAAAAT37300.013.9865951
GTCGGTT46750.013.8737971
GTCGGAT31700.012.454261
GTTTAAT87400.012.36842251
GCGGTAT28250.012.1451341
TATACGC8950.012.0782137
GGAAAAT166300.011.8701151
TACGCTA8350.011.8203599
GTTTTTT262050.011.6580821
GTTTATT99400.011.3008051
GTTTTAT137700.011.2977491
CGAAGAT86600.011.2886841
GTTCGAA41950.010.9797381
CGCGTTT16300.010.6687121
TTTTTTC324400.010.3881022
Produced by FastQC (version 0.11.5)