FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22768890
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG147450.041.62903294
CAAGTCG109350.025.96067894
TAAGTCG34800.020.39367994
GAAGTCG134300.019.80789294
CGAAAAT41850.013.2520911
TTAGCGA19600.012.2295927
GTCGGTT45150.012.1794021
GTTTTAT138600.011.5634931
AAAGTCG62250.011.55180794
CTTTAAT92900.011.5349861
CGAAGAT89150.011.492991
GGAAAAT168350.011.4743111
CGCGAAC19350.011.4160211
CTCAGAT169500.011.0637181
CTTTATT150000.011.0606671
TACGCTA8953.6379788E-1211.0279339
CGAATCG15700.010.777073
GGAAAAC149200.010.4899461
GTTTTTT261850.010.4823371
GCGGTAT29000.010.3724151
Produced by FastQC (version 0.11.5)