FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23368093
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG140800.058.583194
CAAGTCG108000.031.81203794
TAAGTCG35750.028.92307794
GAAGTCG156800.023.29017894
TTTTTTC380350.015.5945842
AAAGTCG59050.015.44115294
GTTTTTT295750.015.3038041
TTTTTTG398550.013.6913822
GTTTAAT92050.013.2753941
CGAAAAT37850.012.9141351
AATCCAC118850.012.5755165
AATCGTA17250.012.260875
GTTTTAC61850.012.0064671
CGAATCG15750.011.9365083
CGCAATT22850.011.9299781
AAATCCA186950.011.8159944
GTTTTCT212500.011.7887061
TTTTTTA363300.011.6691452
GTTTTAT163500.011.6422021
GAAAAAT207950.011.5946151
Produced by FastQC (version 0.11.5)