FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23368093
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG161050.050.39987694
TAAGTCG36450.031.20439194
CAAGTCG122700.028.03912294
GAAGTCG149100.022.69617794
AAAGTCG62950.016.7990594
GTTTTTT273850.013.5413551
CGGATAT24800.013.4556461
CGAAAAT47600.013.1323541
CGAAAAC37250.012.86981
CGCAAAC49200.012.41871
AAATCGA29850.012.2814074
CGGAAAT44050.012.2701481
GCCGAAT51000.011.796081
AAAAATC191250.011.1571252
GCGAAAT29950.011.1419041
TATACGC9300.011.118287
GTCGATT20750.011.0987951
CGCAATT23350.011.0706641
CGCGAAC19200.011.0156251
AAATCCA199300.010.91871554
Produced by FastQC (version 0.11.5)