FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21570942
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT36400.014.8489011
GTTTTTT314050.014.576661
CGCGAAC13150.013.9391631
CGCGAAT7600.013.6052641
CGAAAAC31000.013.038711
CGCAAAC41100.012.922141
CGAAAAT44300.012.3069981
AAATCCA213300.012.2292544
AAATCGA35850.012.1924694
AAAAATC227900.012.1263712
CGCAAAT33200.012.0331331
GGAAAAT189100.011.88048651
CGGATAT20850.011.7218231
GTTTAAT96400.011.6524891
CTCAGAT175850.011.6263861
GTTTTAT161100.011.4655491
CGAATCG14450.011.3840833
GTTTTTC182350.011.3150541
AATCGAA26650.011.2870545
TCGGATC36750.011.1265312
Produced by FastQC (version 0.11.5)