FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22682612
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG103600.053.75965594
CAAGTCG86900.028.01611194
TAAGTCG26400.024.92424494
GAAGTCG115100.018.41615994
AAAGTCG47550.014.233438594
TTAGCGA19550.012.020468
CGCGTAT3802.5898774E-411.1315781
GGCGAAT43100.011.0139211
CGTATCG16100.010.5093173
GTCCGAT15800.010.4113921
GTCGGTT47300.010.3340381
TAACGCA22200.010.26801852-53
TCGGATT24500.010.1673472
GGGAAAT166300.010.1178591
GTCGGAT26800.09.8208961
CGCGGAT23700.09.7172991
GGCGATT40500.09.6320991
CCCGTAT27650.09.5189881
GCCGGTT52250.09.4449771
GTCGTAT11002.1827873E-119.4000011
Produced by FastQC (version 0.11.5)