FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22682612
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127250.043.58349694
CAAGTCG106350.024.83685994
TAAGTCG27750.024.0504594
GAAGTCG114300.017.4348294
AAAGTCG52850.012.361494
GGCGATT37150.012.1453561
CGCGGAT27600.011.751
TACCGTA18850.010.9708229
AATCGTA21150.010.8888895
ATACGCG3500.00163045510.7428576
GTCCGAT14100.010.6666671
GGGAAAT155950.010.4578391
GTCGAAT12100.010.0991741
CGGAAAT37350.010.0669341
GCGGTAT23950.010.0083511
GTCGTTT22750.09.9164841
CGCCAAT34000.09.6764711
ATCGTAT17550.09.6410266
GTTCGAT27850.09.619391
GGCGAAT39750.09.5773581
Produced by FastQC (version 0.11.5)