FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21595838
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125800.060.11367494
CAAGTCG100250.030.98952594
TAAGTCG37600.026.12594
GAAGTCG135650.025.05049794
GTTTTTT282500.014.9235391
AAAGTCG48100.014.55925294
TTTTTTC362650.013.4396812
GTTTTAC71300.013.1178121
CGAAAAT33750.013.0903691
TACGCTA9000.013.0555559
CGAATCG15300.012.9019613
CGAAAAC35900.012.5682451
CGCAAAT32450.012.3112481
GTTTAAT88600.011.9887121
GTTTTTC165300.011.8850571
CGACGAT29400.011.6700681
AAAAATC207350.011.6508322
AAATCCA188850.011.4482394
AATCCAC111050.011.3849615
GTTTTAT151650.011.3432251
Produced by FastQC (version 0.11.5)